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rubicon thruplex fd kit  (TaKaRa)


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    Structured Review

    TaKaRa rubicon thruplex fd kit
    Rubicon Thruplex Fd Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rubicon+thruplex+fd+kit/ThruPLEX+DNA-Seq+HV+PLUS+Kit/pmc10618745-161-16-20
    Average 94 stars, based on 7 article reviews
    rubicon thruplex fd kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
    Article Snippet: Data from H3K27ac ChIP-seq were added to the previously published data ( ) based on patient samples being processed exactly as described in ref. using the same antibody (Abcam, ab4729). .. Sequencing libraries were prepared using 0.5 to 10 ng of ChIP or input DNA with the Rubicon ThruPLEX FD Kit (Takara) using the manufacturer's recommended protocol. .. Libraries were then size selected in the range of 240 to 360 bp using a Caliper LabChIP XT DNA 750 Kit (PerkinElmer).

    Chromatin Immunoprecipitation:

    Article Title: Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
    Article Snippet: Data from H3K27ac ChIP-seq were added to the previously published data ( ) based on patient samples being processed exactly as described in ref. using the same antibody (Abcam, ab4729). .. Sequencing libraries were prepared using 0.5 to 10 ng of ChIP or input DNA with the Rubicon ThruPLEX FD Kit (Takara) using the manufacturer's recommended protocol. .. Libraries were then size selected in the range of 240 to 360 bp using a Caliper LabChIP XT DNA 750 Kit (PerkinElmer).

    Generated:

    Article Title: Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
    Article Snippet: Approximately 2 × 10 6 PWR1E, RWPE1, 22Rv1, LNCaP, VCaP, DU145, and PC3 cells were used to perform H3K9me3 (Abcam, ab8898) ChIP-seq as described in ref. . .. Libraries were generated using the Rubicon ThruPLEX FD Kit (Takara) using the manufacturer's recommended protocol. .. Libraries were then size-selected in the range of 150 to 360 bp using AMPure XP beads (Beckman Coulter, A63881) and sequenced with paired-end 50-bp reads to reach 45 million total mapped reads per replicate as recommended by ENCODE guidelines.

    Article Title: Transposable Elements Are Co-opted as Oncogenic Regulatory Elements by Lineage-Specific Transcription Factors in Prostate Cancer
    Article Snippet: Finally, DNA was extracted using the MinElute Kit (Qiagen). .. Libraries were generated using the Rubicon Thruplex FD Kit (Takara) using the manufacturer's recommended protocol. .. Libraries were then size selected in the range of 150 to 360 bp using AMPure XP beads (Beckman Coulter, A63881) and sequenced with paired-end 75-bp reads to reach up to 40 million read pairs per sample.



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